Growth of single-stranded DNA phages in replication mutants of Escherichia coli

Abstract

Host capacity for growth of single-stranded DNA phages was investigated with several replication mutants of E. coli. In dnaL708, dnam709 and dnaS707 mutants, multiplication of phiK was not restricted even at 42 degree C. In dnaM710 cells, however, growth of phiK was severely effected at 42 degree C but not at 33 degree C. Upon infection of phiK, parental replicative form was synthesized at the restrictive temperature, whereas subsequent step (replication of progeny replicative form) was blocked in the dnaZ strain. Growth of phiX174 and alpha3, as tested by transfection, was also thermosensitive in the dnaM710 mutant but not in the dnaL708, dnaM709 and dnaS707 strains. In contrast with lambda, microvirid phages could grow in E. coli cells bearing the groPC259, groPC756 or seg-2 mutation.