Abstract
PREVIOUS work from this laboratory1 has described a technique for obtaining uniform and quantitative inocula of the Gram-positive filamentous anaerobe Actinomyces israelii. It has also been shown2–4 that the medium of Howell and Pine5 could be simplified to give a chemically defined medium containing inorganic salts (including ammonium sulphate), glucose, glutathione, seven L-amino-acids (serine, cysteine, leucine, isoleucine, lysine, tryptophan and glutamic acid) and five growth factors (biotin, inositol, pyridoxal, nicotinic acid and riboflavin) which would support growth of a single strain of A. israelii in repeated serial culture. Further work6 has described some of the chemical and physical changes which occur in a series of defined media during growth of the same Wills strain of A. israelii.
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