Abstract
THERE are few reports of rat tissue cultures producing growth comparable to that from other mammalian species or from chick explants. Martinovitch1, working with rat pituitary and rat ovary in watch-glass cultures, was able to keep the tissues alive in vitro for a considerable length of time; but cell outgrowth was comparatively slight. Abercrombie and Harkness2 cultured regenerating rat liver. Fibro-blasts or bile duct epithelium rarely grew from normal liver cultures, but appeared sporadically in cultures of regenerating material, the outgrowth varying with the state of liver regeneration. Only lymphocytes and granulocytes occurred in cultures regularly and in similar numbers regardless of the state of regeneration. The only case of rat pituitary tissue producing vigorous growth in vitro is that reported by Haymaker and Anderson3.
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