Growth of probiotics for poultry in glucose yeast pepton media, production of biomass, viability and stability after encapsulation with spray drying

Abstract

This study aimed to determine specific growth rate and biomass cells production of probiotics bacteria isolated from Indonesian native chicken gastroitestinal tract. Three bactrias manely Lactobacillus murinus Ar-3, Streptococcus thermophilus Kp-2 and Pediococcus acidilactici Kd-6 were grown in glucose yeast peptone (GYP) medium. A mixture of probiotic bacteria base on rasio of specific growth rate were employed to the experiment for biomass production study. Fermentation was conducted in 2 litres bioreactor, and encapsulation was carried out by centrifugation. Coating process was done by skim milk/maltodextrin medium and dried with spray drying. The results showed that L. murinus Ar-3 had a generation time of 3.25 ± 0.006 hours, S. thermophilus Kp-2 3.68 ± 0.1 hours and P. acidilactici Kd-6 3.74 ± 0.04 hours, thus ratio of generation time was 1 : 1.18 : 1.16. The growth of mixture of probiotic showed the same pattern. Its cells were harvested at maximum cell yield 9.33±0.12 log 10 cfu/ml was achieved. The fermentation effiency was 51 %, the viability of mixed probiotic culture were 10.12 log 10 cfu/ml before spray and 9.12 log 10 cfu/g after spray drying or 90.11%. The stability of encapsulated probiotics in room temperature during 21 days strorage was 90.89%.