Abstract
The introduction of biodiesel to diesel may allow the fuel to be more susceptible to microorganism growth, especially during incorrect storage. To evaluate the effect of adding biodiesel in pure diesel on the growth of Paecilomyces variotii, microcosms containing pure diesel (B0), blend diesel/biodiesel (B7) and pure biodiesel (B100) were used. In microcosm with minimal mineral medium and B0, B7 or B100, after 60 days, the biomass (dry weight) formed at interface oil-water in B7 and B100 was significantly higher when compared to that of B0. Infrared analysis showed reduction of the carbonile fraction in B7 and B100 suggesting formation of intermediate compounds in B7. To monitor possible contamination of fuel storage tank by P. variotii samples were collected and analysed by specific-PCR assay for detection of P. variotii spores in the aqueous phase. This method was able to detect a minimum of 103 spores ml-1, corresponding to 0.0144 ng µl-1 of DNA. Specificity was tested against Aspergillus fumigatus and Pseudallescheria boydii.
Highlights
The microbial contamination of stored fuels, mainly diesel oil, is a major problem in refineries and distribution systems (Bento and Gaylarde, 1996, 2001; Bento et al, 2004).Many factors, such as the presence of water in the bottom of the tanks during storage, have been cited as increasing microbial growth in the systems and can lead to blockingBraz
The growth of P. variotii in B0, B7 and B100 with different inoculum concentrations over a period of 60 days is shown in Figures 1, 2 and 3
Experiments carried out in this study showed the significant production of biomass when the oily phase was blend B7 and with pure biodiesel, due to the ability of P. variotii to develop especially when biodiesel is present
Summary
The microbial contamination of stored fuels, mainly diesel oil, is a major problem in refineries and distribution systems (Bento and Gaylarde, 1996, 2001; Bento et al, 2004).Many factors, such as the presence of water in the bottom of the tanks during storage, have been cited as increasing microbial growth in the systems and can lead to blockingBraz. The microbial contamination of stored fuels, mainly diesel oil, is a major problem in refineries and distribution systems (Bento and Gaylarde, 1996, 2001; Bento et al, 2004). Many factors, such as the presence of water in the bottom of the tanks during storage, have been cited as increasing microbial growth in the systems and can lead to blocking. Bücker et al (2011) reported that Paecilomyces sp produced the highest biomass in biodiesel blends, while Aspergillus fumigatus grew best in pure biodiesel
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
