Abstract
The secretion of CEA into the blood of athymic mice was studied with 4 sublines of human colonic adenocarcinoma cell lines, HT 29 and SLu. Growth curves based on tumour volume (caliper measurements) or tumour mass (weight) correlated with a concomitant increase of serum CEA during the logarithmic growth phase, but showed a marked dissociation when the growth rate slowed down. In the logarithmic growth phase doubling times between 2 and 6 days were calculated and about 6-7 doubling times passed until the shift in the growth rate was observed, independently of the sublines transplanted. Constant increases of CEA between 0.03 and 0.45 microgram/l serum per mm3 increase of tumour volume, depending on the sublines, were recorded during the logarithmic growth phase. Sublines releasing high amounts of CEA in vitro (cell culture) retained this characteristic in vivo. Correlation between tumour volume and tumour mass or serum CEA showed correlation coefficients of 0.820-0.977 during the logarithmic growth phase.
Highlights
Summary.-The secretion of carcinoembryonic antigen (CEA) into the blood of athymic mice was studied with 4 sublines of human colonic adenocarcinoma cell lines, HT 29 and SLu
Growth curves based on tumour volume or tumour mass correlated with a concomitant increase of serum CEA during the logarithmic growth phase, but showed a marked dissociation when the growth rate slowed down
Since numerous experiments with xenografted human malignant tumours into thymus-deficient mice have been published from which it is evident that human tumour transplants in athymic mice can retain certain characteristics of the original tumour cell line (Pesce et al, 1977; Helson et al, 1975; Dipersio et al, 1980; Papsidero et al, 1981)
Summary
Orbital sinus yielding 200-250 ,ul serum per animal. Mice.-Inbred STU mice (Committee on CEA determination.-Serum CEA constandardized nomenclature for inbred strains centrations were determined with the CEA of mice, 1968) were used to derive inbred Roche RIA test kit. The nude mice were kept under used throughout the experiments. CEA pathogen-limited conditions according to concentrations > 20,ug/l were measured after the guide-lines given by the Committee on appropriate dilution of the sera. Control care and use of the "nude mouse" (1976). Nude mouse serum did not contain measurable.
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