Abstract
Attempts were made to expect continuous growth of normal human cervical epithelial cells in order to study possible oncogenic effects of Herpes Simplex Virus type 2 (HSV-2). Surgical specimens from patients undergoing hysterectomy and other operation for non-malignant pathology were obtained and cultured with a “sandwich method.” The life span of normal endo- and ectocervical cells was limited only to 2 weeks. The monolayer cells treated with both Feeder layer and Epidermal Growth Factor (EGF) showed longer life for several months. The growing cells under the above condition infected with HSV-2 showed less degree of cornification and survived in soft agar. The normal human cervical epithelial cells required both Feeder layer and EGF, but no oncogenic transformation was obtained with HSV-2 infection.
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