Growth of fungi and mycotoxin production on cheese under modified atmospheres

Abstract

The use of modified atmospheres to prevent fungal growth and mycotoxin production in cheese was evaluated. Eight fungal species: Mucor plumbeus, Fusarium oxysporum, Byssochlamys fulva, B. nivea, Penicillium commune, P. roqueforti, Aspergillus flavus and Eurotium chevalieri were inoculated onto cheese and incubated under conditions of decreasing concentrations of O 2 (5% to <0.5%) and increasing concentrations of CO 2 (20–40%). Fungal growth was measured by colony diameter and ergosterol content. All fungi examined grew in atmospheres containing 20% and 40% CO 2 with 1% or 5% O 2, but growth was reduced by 20–80%, depending on species, compared with growth in air. The formation of aflatoxins B 1 and B 2, roquerfortine C and cyclopiazonic acid was greatly decreased but not totally inhibited in these atmospheres. At 20% or 40% CO 2 with <0.5% O 2, only B. nivea exhibited growth, which was very slow. Growth of F. oxysporum, B. fulva, P. commune and A. flavus showed good correlations between colony diameter and ergosterol content. However, for the other species correlations were inconsistent.