Growth of Chinese hamster ovary (CHO) cells in the presence of MMC in low-serum medium

Abstract

Chinese hamster ovary (CHO-WBLT) cells growing in McCoy's 5a with 10% fetal bovine serum (FBS) were adapted to 0.5% FBS in CHO-1 Complete Media System, a serum-free medium from Ventrex. Cells in these two media were exposed to 10 −7 M and 10 −8 M mitomycin C (MMC) for 24 h. Comparison of cell growth over 10 days showed that cells in 0.5% serum proliferate, though at a slower rate than cells in 10% serum. Treatment with MMC revealed that at 10 −7 M, MMC is cytotoxic to cells in both the media; at 10 −8 M, MMC is non-cytotoxic to cells in both media.