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Abstract
TECHNIQUES now exist for obtaining clonal growth in semi-solid agar of mouse neutrophil, macrophage, eosinophil, erythropoietic and megakaryocytic colonies1–5. Previous attempts to obtain colony formation by mouse lymphoid cells, however, were unsuccessful and cultured lymphocytes died very quickly, even in the presence of known lymphocyte mitogens. Mercaptoethanol and related substances have been shown to increase lymphocyte proliferation in liquid cultures6 and colony formation in agar by mouse plasmacytoma cells7. Based on these observations, attempts were made to obtain clonal growth of mouse lymphoid cells in agar medium supplemented with 2-mercaptoethanol.
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