Growth of Arboviruses in Arthropod Tissue Culture

Abstract

Publisher Summary The growing interest in the arbovirus infections of man and domestic animals has stimulated research in cell cultures from arthropods of medical importance, especially mosquitoes and ticks, to study the vector–virus relationship at a cellular level. Primary cell cultures derived from ticks have been used more extensively for the study of arbovirus growth than primary cultures derived from mosquitoes. The growth of arboviruses in various mosquito cell lines and particularly in A. albopictus cell line seems to follow a certain pattern. Quantitative assay of the multiplication of viruses in arthropod cell lines have revealed two types of growth pattern: the first in which the maximum titer never exceeds the initial inoculums more than 10-fold, and the second in which the maximum titer reaches more than 100- to 100,000-fold the original inoculurn. In most of the cell lines tested so far for virus growth, a definite decline in the virus titers has been observed during the first 24 hours. This decline was followed by a rise in titer reaching a peak in the majority of cases between day 3 and day 6. After this peak, the virus titer started declining gradually.