Abstract
The morphology ofMycoplasma mobile 163K grown on glass coverslips and Pioloform-coated copper grids in liquid medium was studied by dark-field, interference-contrast, scanning, and negative-contrast electron microscopy. The growth morphology was observed over a period of 5 days. Within this time, three distinct stages could be distinguished: (a) single cells; (b) reproduction, aggregation, and microcolony formation; and (c) degeneration. The single cells were typically flask-shaped. Different modes of reproduction were observed: symmetrical binary division by transverse fission, budding-like processes, and fragmentation. Symmetrical binary division occurred mainly in the first half stage of reproduction, leading to the formation of rosette-like microcolonies, whereas budding and fragmentation took place only in the second half. During degeneration, the flask-shaped cells converted into rounded forms and lost their viability.
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