Abstract
How a collection of single neurons self-organize to form a complex functional system, the neural network, is a fundamental question. Two-dimensional in vitro invertebrate preparations offer an attractive model system to tackle this question due to the large size of the neurons, and their ability to grow in relative isolation as well as to develop elaborate networks. We culture locust neurons, monitor and analyze their morphology and growth process under various density conditions. The neurons actively target neighbor cells, and their structure is affected by neuronal vicinity. As the network forms there is a tendency for simplification of neuronal morphology.
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