Growth, Mg nutrition and photosynthetic activity in Pinus radiata: evidence that NaCl addition counteracts the impact of low Mg supply

Abstract

We investigated effects of low Mg and moderately raised NaCl, as occurs in plantations irrigated with tertiary municipal effluent in New Zealand, on growth, Mg nutrition and photosynthetic activity of Pinus radiata D. Don seedlings grown in nutrient solutions with a Perlite medium. Seedlings were grown with either sufficient (0.35 mM; H[Mg]) or limited (0.033 mM; L[Mg]) Mg supply, without NaCl or with NaCl addition (8.7 mM; +[NaCl]). After 30 weeks, seedlings grown at L[Mg] displayed severe Mg deficiency symptoms, and had significantly less biomass than those at H[Mg]. While NaCl addition had an adverse effect on seedling growth at H[Mg], it increased growth at L[Mg]. The +[NaCl] treatment greatly increased the Mg uptake rates, which were associated with increased stomatal conductance and increased root to shoot ratio. Magnesium deficiency reduced the rates of light-saturated photosynthesis and stomatal conductance, but not the quantum efficiency of photosystem II, which was reduced mainly by the +[NaCl] treatment, especially at H[Mg]. Our study clearly indicated that NaCl addition could counteract the impact of low Mg supply by enhancing Mg uptake from the rooting medium.