Abstract
ABSTRACTEscherichia coliO157:H7‐specific antibodies (immunoglobulin Y [IgY]) were isolated by the water‐dilution method from the egg yolk of chickens that were immunized withE. coliO157:H7 whole cells. The specific‐binding activity of IgY againstE. coliO157:H7 as determined by the enzyme immuno assay showed high levels of activity against bacterial whole cells. IgY binding activity was further demonstrated to have an inhibitory effect onE. coliO157:H7 growth in a liquid medium. The antibacterial function of IgY appeared to result from the interaction of IgY with surface components ofE. coliO157:H7, as proven from observation of immunofluorescence and immunoelectron microscopy.
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