Abstract
Growth inhibition effect of different concentration of distilled water extract and four polar organic solvent (methanol, acetone, ether and chloroform) extracts ofUlva pertusa on three typical red tide microalgae (Heterosigma akashiwo, Alexandrium tamarense andProrocentrum micans) were investigated. Liquid-liquid fractionation and HPLC analysis for methanol extract ofU. pertusa were carried out. Growth of the three microalgae was significantly inhibited by the distilled water extract ofU. pertusa at relatively higher concentration. However, the cells of the three microalgae did not die completely even at high concentration. Methanol extract ofU. pertusa showed the highest growth inhibition on the three microalgae, and all the cells of the three microalgae were killed at relatively high concentration. The other three organic solvent extracts ofU. pertusa had no apparent effect on the three microalgae. The results of bioassays and HPLC analysis suggested that the inhibitory substances inU. pertusa to the microalgal growth had relatively high polarities.H. akashiwo was the most sensitive one whileA. tamarense was the most tolerant one to the growth inhibitory substances.
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