Abstract

The objective of this study was to determine the maximum ammonium source concentration tolerated by Selenomonas ruminantium and its metabolic response to high ammonium source concentrations. The ammonia-nitrogen half-inhibition constant (K i) in defined basal medium was 239 mabetm for NH4Cl, 173 mabetm for NH4OH, 153 mabetm for (NH4)2SO4 and 110 mabetm for NH4HCO3. Reduction in continuous culture maximal growth rate was similar to the reduction in the batch culture logarithmic growth rate for the respective NH4Cl concentrations. Cell yield when expressed as Y ATP decreased for 150 and 200 mabetm NH4C1. the NH3-N K i estimates are in line with inhibiting concentrations observed for other bacteria and suggest that energy efficiency is reduced when the NH3-N concentration is increased.

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