Growth inhibition of mice mammary carcinoma cell line with green synthesized Magnetic Iron Oxide Nanoparticles

Abstract

Mammary adenocarcinoma represents one of the most cancers that effect woman in Iraq and worldwide. Finding a definitecure for this type of cancer is a target of considerable research in many laboratories over the globe. In this study, a greensynthesized magnetic iron oxide nanoparticles (MNP) was used to specify their ability to inhibit the growth of mice mammarycarcinoma cell line (AMN3), it comes as a preliminary study of wider ongoing research. After successfully synthesizing magneticiron nanoparticles, it employed in different concentrations to expose AMN3 cells for 24 and 48 hours. The viability of theexposed cells was determined using standard MTT assay. The inhibition concentration 50 was determined afterward and usedto test the possibility of inducing apoptosis in the carcinoma cells using a mixture of fluorescent stains acridine orange andpropidium iodide. Results indicated that green synthesized MNP can inhibit the growth of mammary adenocarcinoma cell lineup to 79% and 89% after 24 and 48 hr of incubation with 1000μg/ml. The IC50 of MNP was capable of inducing apoptosis inthis cell line as shown by fluorescence microscope. The standard hemolysis assay used to test the reaction between the greensynthesized magnetic oxide nanoparticles and normal cells. The IC50 of AMN3 cells growth inhibition only induce 14% ofRBC hemolysis, while the percentage induced by negative control was 10%. In conclusion, green MNP have an effect uponcancer cells greater than normal cells. This material must tested against other types of cancer and normal cells to verify itsaction and study mechanisms of apoptosis induction in detail.Key words: Magnetic iron oxide nanoparticles, AMN3, green synthesis, nanotherapy.