Growth-inhibition in hepatocelluar carcinoma caused by lentivirus mediated dual siRNA targeting MAT2A and MAT2B

Abstract

Objective To investigate the suppressive effects caused by lentivirus mediated dual siRNA targeting MAT2A and MAT2B expression in hepatic cancer HepG2 cells. Methods Two siRNAs targeting MAT2A and MAT2B respectively were cloned to one lentivirus work vector simultaneously. Work vector and three package plasmids were co-transfected into 293T cells with the help of lipofectamine2000. Lentivirus was collected after 72 h, and was added to the cultured HepG2 cells. Cells were counted every day and total mRNA was extracted and underwent RT-PCR. Apoptosis was figured out with flow cytometry. The changes of S-adenosylmethionine in HepG2 cells were also studied. Results Dual small interfering RNA targeting MAT2A and MAT2Bs imultaneously was constructed successfully. Expression of MAT2A and MAT2B was suppressed by 89.5% and 97.8% respectively. Cell growth was inhibited by 50%. Intra-cellular S-adenosylmethionine level was increased over 2 times. Cell apoptosis was induced. Conclusion Dual small interfering RNA mediated by lentivirus can simultaneously inhibit the expression of MAT2A and MAT2B, resulting in growth-inhibition of hepatic cancer cells. MAT2A and MAT2B may be new thera-py targets for hepatocelluar carcinoma in the future. Key words: Carcinoma,hepatocelluar ; Lentivirus ; RNA interfering