Abstract
BackgroundCold carcass weight (CW) and longissimus muscle area (EMA) are the major quantitative traits in beef cattle. In this study, we found several polymorphisms of growth hormone-releasing hormone (GHRH) gene and examined the association of polymorphisms with carcass traits (CW and EMA) in Korean native cattle (Hanwoo).ResultsBy direct DNA sequencing in 24 unrelated Korean cattle, we identified 12 single nucleotide polymorphisms within the 9 kb full gene region, including the 1.5 kb promoter region. Among them, six polymorphic sites were selected for genotyping in our beef cattle (n = 428) and five marker haplotypes (frequency > 0.1) were identified. Statistical analysis revealed that -4241A>T showed significant associations with CW and EMA.ConclusionOur findings suggest that polymorphisms in GHRH might be one of the important genetic factors that influence carcass yield in beef cattle. Sequence variation/haplotype information identified in this study would provide valuable information for the production of a commercial line of beef cattle.
Highlights
Cold carcass weight (CW) and longissimus muscle area (EMA) are the major quantitative traits in beef cattle
By pair-wise linkage analysis with DNA from the 24 unrelated Korean cattle, which were used for direct sequencing, we have found that two sets of polymorphisms were in absolute linkage disequilibrium (LD) (|D'| = 1 and r2 = 1)
We found that polymorphisms in growth hormonereleasing hormone (GHRH), one of the predominant regulators of growth hormone (GH) releasing [16], were associated with carcass yield traits (CW and EMA) in Korean native cattle
Summary
Cold carcass weight (CW) and longissimus muscle area (EMA) are the major quantitative traits in beef cattle. We found several polymorphisms of growth hormonereleasing hormone (GHRH) gene and examined the association of polymorphisms with carcass traits (CW and EMA) in Korean native cattle (Hanwoo). There have been several reports of association between quantitative traits in cattle, such as growth performance and carcass merit, and polymorphisms in the GH gene [4,5,6]. We examined GHRH as one of candidate genes in meat production. We performed extensive screening of GHRH by direct sequencing to detect polymorphisms and examined genetic association with the carcass traits. We present 12 polymorphisms identified in GHRH and the results of an association study with meat quantity in Korean native cattle (Hanwoo)
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