Growth hormone influence on functional status of cord blood-derived dendritic cells

Abstract

To detect the effect of functional status and immunophenotype of dendritic cell modified with growth hormone (GH) from by cord blood. Monocyte was isolated from cord blood, DCs were induced with IL-4 & GM-CSF and modified with different concentration of GH. The morphology, count and growth status of DC were observed appearance. And the immunophenotype of DC was detected with flow cytometry. IL-12 in supernatant was measured with ELISA. The immuno-activity was determined with autologous mixed lymphocyte reaction. Mature DC were induced from adherent lymphomonocyte under the action of IL-4, GM-CSF and GH of different densities (5, 25, 100 ng/ml). GH increased the positive expression of CD83 (31% +/- 4%, 34% +/- 4%, 34% +/- 5%), CD80 (26% +/- 4%, 29% +/- 6%, 30% +/- 6%)and HLA-DR (45% +/- 8%, 49% +/- 6%, 50% +/- 5%) (P < 0.05), enhanced the ability of secreting IL-12 (P < 0.05), and boosted significantly the immuno-activation of DC to autologous lymphocyte (P < 0.05). GH could promote the differentiation, maturation and the expression of costimulatory molecules, increase the IL-12 production in DC and enhanced the ability of DC to stimulate the proliferation of lymphocyte within a certain dose range.