Abstract
ObjectivesTo study the expression of growth factors in the regulation of tissue repair after peritoneal damage tissue response to peritoneal damage.MethodsExperimental study in 35 male Wistar rats determining the evolution over time of the tissue response to aseptic peritoneal damage. A standardized bowel and peritoneal lesions were created in the right lower quadrant by laparotomy. Then, tissular expression of growth factors was evaluated by multiplex polymerase chain reaction at seven timepoints between 6 h and 30 days, postoperatively.ResultsTissular responses of granulocyte-stimulating factors (Csf2, Csf3), connective tissue growth factor (Ctgf), epidermal growth factors and receptor (Egf, Egfr), fibroblast growth factors (Fgf2, 7 and 10), heparin binding EGF-like growth factor (Hbegf), hepatocyte growth factor (Hgf), insulin-like growth factor-1 (Igf1), mitogenic transforming growth factors (Tgfa, Tgfb1, Tgfbr3), and vascular endothelial growth factor A (Vegfa) were biphasic with a first expression peak at day 3, followed by a more pronounced peak at day 14.ConclusionsWe observed a long-lasting, widespread response of tissular growth factors for at least two weeks after peritoneal damage. To be clinically effective, the prophylaxis of postoperative adhesions might be needed for an extended period of time.
Highlights
In recent decades, growth factors have attracted a lot of attention in the study of the pathogenesis of various diseases and conditions because they play a crucial role in the processes of growth, development, and maintenance of cell populations
Tissular expression of growth factors was evaluated by multiplex polymerase chain reaction at seven timepoints between 6 h and 30 days, postoperatively
Growth factors have attracted a lot of attention in the study of the pathogenesis of various diseases and conditions because they play a crucial role in the processes of growth, development, and maintenance of cell populations
Summary
Growth factors have attracted a lot of attention in the study of the pathogenesis of various diseases and conditions because they play a crucial role in the processes of growth, development, and maintenance of cell populations. Growth factors are biologically active polypeptides that function as regulatory signals controlling cell proliferation and differentiation, and they promote cell survival [1, 2]. Most growth factors are multipotent and can affect various cell types. Their names often reflect specific cellular types, their action might not be limited to this cell population. Vascular endothelial growth factor (VEGF) stimulates vascular growth. Still, it is involved in the proliferation, migration, differentiation, and mobility of fibroblasts, both in physiological and reparative regeneration processes [3,4,5]. Fibroblast growth factor 2 (FGF2) can act as an activator of angiogenesis [7, 8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
