Abstract
Summary Rapidly growing, fine suspension cultures were established from hypocotyl pieces of Lycopersicon esculentum cv. Lukullus and from cotyledons of L. peruvianum. They are characterized with respect to their growth pattern, nutrient consumption as well as uptake and incorporation of precursors of nucleic acid and protein synthesis. The doubling time of these tomato suspension cultures in a modified Murashige-Skoog medium is ~ 24 h. Analyses of the macronutrient consumption during the growth cycle (carbohydrate, nitrate, ammonia, phosphate) revealed a phosphate-limited growth with exponential increase of cell number and dry weight during 3–5 d. Normally 3% sucrose is used as carbon source; but glucose is suitable as well. In L. esculentum suspensions sucrose is rapidly hydrolysed by a cell wall bound acid invertase, and the liberated glucose and fructose are consumed consecutively. Long-term cultures from both tomato species exhibit very low morphogenetic potential. With L. peruvianum green, heterogeneous suspensions can be obtained after prolonged passages on cytokinin-free media containing indole acetic acid as the sole growth hormone.
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