Growth conditions, physiological properties, and selection of optimal parameters of biodegradation of anticancer drug daunomycin in industrial effluents by Bjerkandera adusta CCBAS930

Teresa Korniłłowicz-Kowalska,Kamila Rybczyńska-Tkaczyk

doi:10.1007/s10123-019-00102-3

Teresa Korniłłowicz-Kowalska, Kamila Rybczyńska-Tkaczyk

Open Access

https://doi.org/10.1007/s10123-019-00102-3

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Abstract

The study characterizes the anamorphic Bjerkandera adusta strain CCBAS 930, including growth conditions, physiological properties, and enzymatic activities related to basic metabolism and specific properties coupled with the fungal secondary metabolism. It was established that the fungus grows in a wide pH range (3.5–7.5), up to 3% of salt concentration and a temperature of 5–30 °C. Media rich in natural organic components (potato, maize extracts, whey) are optimal for biomass propagation. Minimal media, containing mineral salts and glucose as well as static growth conditions, are required to obtain idiophasic mycelium, equivalent to the secondary metabolism of the fungus. Of the 7 complex C, N, and energy sources tested, the strain did not utilize only fibrous cellulose. Lipolytic activity reached the highest values of the enzymatic activities corresponding to those capabilities. The specific properties of strain B. adusta CCBAS 930 determined by the production of HRP-like peroxidase were related to the decolorization and biodegradation of anthraquinone derivative daunomycin. The decolorization of 30% of daunomycin effluents occurred most rapidly in iso-osmotic medium and non-enriched with nitrogen, containing 0.25% glucose, pH = 5.0–6.0, and 25–30 °C. In agitated cultures, the strain decolorized solutions of daunomycin by biosorption, which coincided with the inhibition of aerial mycelium production and HRP-like biosynthesis. Based on knowledge, potential and real possibilities of using the strain in bioremediation of colored industrial sewage were discussed.

Highlights

Daunomycin is a glycoside containing an amino sugar and 3 anthracyclic rings and a metoxylic group attached to the first ring
Anamorphic B. adusta CCBAS 930 was isolated from the black earth soil (Pheozemes, FAO classification) as a strain decolorizing daunomycin, remaining in the post-production agarized Streptomyces peucetius mycelium, which is a waste of the pharmaceutical industry
The effect of medium decolorization, as a result of biosorption was visible already after 7 (10% effluent) and 10 (20% effluent) days of submerged culture (Table 6). This phenomenon has never been observed in stationary cultures, in which enzymatic daunomycin decolorization occurred in the medium

Summary

Introduction

Daunomycin is a glycoside containing an amino sugar (glucosamine) and 3 anthracyclic rings (anthraquinonic compound) and a metoxylic group attached to the first ring. The aromatic component is responsible for biological activity and at the same time for the color (red pigment). The cytotoxic mechanism of action of daunomycin—as an inhibitor of DNA biosynthesis—involves the intercalation of this antibiotic into a newly synthesized polynucleotide chain, which leads to replication suppression (Zhang et al 2013). Anthracycline antibiotics are obtained from the mycelium of Streptomyces The level of anthracycline antibiotics in surface water ranges from ng/L to μg/L (Kolpin et al 2002; Ashton et al 2004; Lenz et al 2007; Mompelat et al 2009; Negreira et al 2014)

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