Growth, condition and energy stores of Arctic grayling fry inhabiting natural and artificial constructed Arctic tundra streams

Abstract

Juvenile north-temperate and Arctic fishes are faced with trade-offs between energy allocation to growth and energy storage (primarily lipids) prior to over-wintering. We determined classical morphometric (fork length, body weight and condition factor) and biochemical (whole body triglycerides, muscle RNA/DNA ratio, muscle proteins) measures of growth and condition in individual young-of-the-year (YOY) Arctic grayling ( Thymallus arcticus ). Grayling were collected just prior to over-wintering in late August (approximately 50 days after swim-up) from two natural streams and five locations within a 3.4 km long artificial stream constructed as a fish habitat compensation project and diversion channel for the diamond mining industry in Northwest Territories, Canada (64°45′N). Fork lengths, body weights and whole body triglyceride levels in grayling collected from all sites along the artificial stream were significantly lower than fish collected from one of the natural streams. Condition factor (weight-at-length) was not different among grayling collected from natural and artificial streams. Muscle proteins were lower in grayling collected from four sites along the artificial stream compared to the natural streams. In contrast, muscle RNA/DNA ratios were greater in grayling collected from two sites in the artificial stream compared to natural streams. There were no consistent differences in any variable among grayling collected at the five artificial stream sites or among grayling collected from the two natural streams. The higher RNA/DNA ratios and lower fork lengths, whole body triglycerides and muscle proteins in grayling inhabiting the artificial stream are consistent with energy still being primarily allocated to growth in these fish at this late stage of summer. Individuals that are both larger and possess greater energy storage in the form of triglycerides are more likely to survive the long over-wintering period at this latitude. Our results suggest that YOY grayling using the artificial stream as nursery habitat will likely face increased over-winter mortality, thus raising concerns over the use of fish presence, spawning and rearing as criteria for the initial success of artificial streams as habitat compensation measures in Arctic tundra regions. Further research is needed to determine the potential consequences of reduced size and energy storage in juvenile fishes in order to assess the viability of stream fish habitat compensation and restoration projects associated with industrial development in Arctic tundra regions.