Abstract
Spontaneous antibiotic-resistant strains of Bradyrhizobium, from six parent strains isolated from Desmodium intortum, were compared with their parents for changes in ecologically-important characteristics. Strains resistant to 30 μ g rifampicin ml −1 and 500 μg streptomycin ml −1 were serologically homologous with their parents in gel-immunodiffusion tests against antisera for the parent strains. They had similar growth and rhizosphere colonizing abilities, but were not equally effective in N 2 fixation or equally competitive for nodule formation on D. intortum. Such variation suggests, firstly that a single colony isolate should not be used to represent a parent strain. Secondly, if single colony isolates are used they should be considered as strains for their individual characteristics.
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