Abstract
The growth of Trypanosoma cruzi in two liquid media has been studied. Both media contain NaCl, KCl, phosphate buffer, glucose, calf serum and hemoglobin. In addition, the medium that supports faster growth contained liver infusion and tryptose (LIT medium), while the other contained lactalbumin hydrolyzate. T. cruzi multiplies in LIT medium with a generation time of 14 hours and a yield of about 6 gm of fresh weight per liter of medium in 4 days of culture. The respiration, biosynthesis of protein, ribonucleic and deoxyribonucleic acids by the flagellates have been studied in different phases of growth of the organisms. The effect of serum and liver infusion concentration as well as the influence of temperature and shaking on the growth rate have been investigated. Data on the chemical constitution of the flagellate in different phases of growth, such as the content of protein, ribonucleic and deoxyribonucleic acids, and the base composition of the nucleic acids are presented.
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