Growth and Welfare of African Catfish (Clarias gariepinus Burchell, 1822) under Dietary Supplementation with Mixed-Layer Clay Mineral Montmorillonite-Illite/Muscovite in Commercial Aquaculture

Abstract

Juvenile African catfish (Clarias gariepinus Burchell, 1822) were reared within two experiments (a research facility and a local catfish farm, E1 and E2, respectively) for 102 d each under commercial recirculating aquaculture conditions. The mixed-layer clay mineral montmorillonite–illite/muscovite (1g557) was applied as a feed additive at concentrations of 0.5% and 2.0%, which were compared with an unsupplemented control (0.0%) over 70 d. For E1, feeding was automatic at night, while E2 was fed manually during the day. The growth and physiological welfare parameters of the fish were monitored, including the mortality, skin lesions, stress responses after confinement (plasma cortisol and glucose), and additional blood parameters. Tendentially, the most efficient growth in both the experiments was observed in the 0.5% groups, which performed slightly better than the controls (E1: 0.8% and E2: 3.2%) despite a lower nutrient content (p > 0.05). In E1, the negative skewness of the leptokurtic distribution also revealed the highest number of larger-sized fish per batch. Mortality was low in all the treatment groups (E1 control/0.5%/2.0%: 3.6%/4.9%/2.9%; E2 control/0.5%: 2.6%/5.5%). After only 29 d in E1, the number of skin lesions per fish decreased significantly (p < 0.05 between each of the 0.5% and 2.0% groups, compared to the control (E1 control/0.5%/2.0%: 1.2/0.8/0.8). In both E1 and E2, the number of lesions per fish decreased even further after 70 d, significantly between the treatment groups and the control (E1 control/0.5%/2.0%: 0.9/0.4/0.5 and E2 control/0.5%: 0.6/0.3). In E1, the cortisol and glucose concentrations increased strongly in all the groups due to the induced stress, whereas this was not evident in E2 based on the different sampling procedure. The additional blood parameters (aspartate aminotransferase, glutamate dehydrogenase, urea, calcium, phosphate, total protein, leucocytes, erythrocytes, hematocrit, cholesterol, triglycerides, sodium, potassium, and chloride) revealed no significant difference between the treatment groups in either experiment, indicating no negative effects of 1g557 on the organs or metabolism of the fish. Supplementation with 0.5% 1g557 in the common commercial feeds for African catfish increases growth performance (p > 0.05), reduces size variance, and supports fish welfare under different commercial aquaculture conditions in the present study.