Growth and survival of self-fertilized and hybridized families of Haliotis discus hannai

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 皱纹盘鲍4个养殖群体杂交子代生长与存活状况比较 DOI: 作者: 作者单位: 1. 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006;2. 上海海洋大学 水产与生命学院, 上海 201306 作者简介: 江海林(1992-),女,硕士研究生,专业方向为贝类遗传育种.E-mail:hailinchiang@foxmail.com 通讯作者: 中图分类号: S966 基金项目: 山东省农业良种工程项目（2017LZQC009）. Growth and survival of self-fertilized and hybridized families of Haliotis discus hannai Author: Affiliation: 1. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为利用杂种优势培育皱纹盘鲍（）优良新品种，本研究以4个不同养殖群体[黄岛（HD）、荣成（RC）、日本（JP）、大连（DL）]的皱纹盘鲍为亲本，设计4×4完全双列杂交，建立了4个自交家系和12个正反杂交家系，在利用微卫星标记进行亲子鉴定的基础上，对各家系的F1在1、5、13、17月龄时的生长性状、杂种优势率、生长速度和存活情况进行比较，分析杂交效应。结果表明，在各个生长阶段均有部分杂交家系与自交家系相比表现出显著的生长优势；HDRC、HDDL和JPDL家系的生长速度较高；HDDL、HDJP、RCDL、JPRC及RCHD家系有着较高的存活率；在杂种优势方面，HDRC、HDDL与DLHD家系在各生长参数与生长速度上有明显的杂种优势，HDDL、RCDL、DLHD家系在存活率上表现出明显的杂种优势。本研究筛选出的具有优势的交配组合，可作为皱纹盘鲍生产上种苗来源的参考，也为利用杂种优势培育皱纹盘鲍新品种提供了基础资料。 Abstract:(abalone, Gastropoda) is an economically important species. However, the rapid development of industrialized and intensive farming, as well as frequent hybridization and inbreeding, are gradually reducing the genetic diversity and parental effective group of hybrid abalone, thereby reducing abalone growth and quality. An effective way to improve abalone quality is to breed a new fast-growing and disease-resistant variety. Cross breeding, which increases the yield and viability of hybrid offspring heterosis, is a traditional breeding method that plays an important role in improving aquatic animals. In the present study, we constructed four self-fertilized and twelve reciprocal hybrid families from four cultured populations (Huangdao, HD; Rongcheng, RC; Japan, JP; and Dalian, DL) using a 4×4 complete diallel cross. Based on paternity testing, which was performed using eight polymorphic microsatellite markers, the growth traits, heterosis rate, growth rate, and survival of the F1 families were compared at 1, 5, 13, and 17 months, and the hybridization efficiency was estimated. Some hybrid offspring exhibited significant growth advantages, when compared to self-fertilized families. The HDRC, HDDL, and JPDL families exhibited greater growth rates than those of other families, and the HDDL, HDJP, RCDL, JPRC, and RCHD families exhibited greater survival rates. Furthermore, the HDRC, HDDL, and DLHD families exhibited significant heterosis in growth parameters and growth rate, and the HDDL, RCDL, and DLHD families exhibited significant heterosis in survival. The heterosis of body weight, body length, and body width of the same family in the same stage and heterosis of the same trait of one family in different stages were found different in the present study, which indicates that hybrid families possess unbalanced and unstable heterosis. Differences in the gene frequency of populations are the basis of heterosis. The results of the present study suggest that there are significant genetic differences among the HD, DL, and RC populations. The superior hybrid offspring identified in the present study can be used as a germplasm reference and provide a basis for breeding new varieties of . 参考文献 相似文献 引证文献