Abstract
Azospirillum brasilense and Arthrobacter giacomelloi were grown in single and mixed succinate-limited continuous cultures at a partial oxygen pressure of 0.01 atm. Growth, viability and survival during nutrient starvation were examined at various dilution rates. At D = 0.05 h-1, Ks values for succinate consumed were calculated. Arthrobacter giacomelloi viability was inversely related to dilution rate whereas Azo. brasilense was directly related. Slightly lower values of viability were obtained in mixed culture, but the ratio between the microorganisms was constant. The survival of Arth. giacomelloi in single culture decreased with increasing growth rate while survival of Azo. brasilense was directly related to dilution rate. Acetylene reduction activity was generally very low in both single and mixed cultures. Respiration rate was also determined and the mixed culture showed an oxygen uptake rate higher than that of single cultures.
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