Abstract
AbstractOnce a hybridoma line has been selected and cloned, it can be expanded and seed stocks cryopreserved for future use. Relatively large amounts of purified MAb may also be required. There are a variety of procedures for this that ensure the establishment of a stable cell line secreting high levels of specific immunoglobulin. High concentrations of antibody can be generated by growing the line in the peritoneal cavity of mice/rats of the same strain as the tumor cell line donor and spleen cell donor. Antibody is secreted into the ascitic fluid formed within the cavity at a concentration up to 10 mg/mL. However, the ascites will contain immunoglobulins derived from the recipient animal that can be removed by affinity chromatography if desired. Several in vitro culture methods using hollow fibres or dialysis tubing (1) have been developed and are commercially available; this avoids the use of recipient mice/rats and contamination by host immunoglobulins, although contamination with culture medium-derived proteins may be a problem.KeywordsHollow FibreAscitic FluidStable Cell LineNormal MediumDialysis TubingThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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