Abstract
ObjectiveThis study evaluated the change and function of the pancreas, and small intestine in relation to growth performance of broilers on diets supplemented with raw soybean meal (RSBM) and protease. Samples of test ingredients and diets, after mixing and prior to being used were also assessed on contents of anti-nutritional factors.MethodsA 3×3 factorial study was used, with three levels of RSBM (commercial soybean meal [SBM] was replaced by RSBM at 0, 10%, or 20%) and protease (0.1, 0.2, or 0.3 g/kg). Each treatment was replicated six times with nine birds per replicate. Birds were housed in cages, in climate-controlled room and fed starter, grower and finisher diets.ResultsLevels of trypsin inhibitors in the diets, containing varying levels of RSBM ranged between 1,730.5 and 9,913.2 trypsin inhibitor units/g DM. Neither RSBM nor protease supplementation in diets significantly affected (p>0.05) the body weight of broilers in the entire periods (0 to 35-d). Increasing the level of RSBM in diets increased the weight of the pancreas at d 10 (p<0.000), d 24 (p<0.001), and d 35 (p<0.05). Increasing levels of RSBM in the diets reduced the apparent ileal digestibility of crude protein (CP), and amino acid (AA) at d 24. Increasing level of RSBM in the diets decreased (p<0.01) pancreatic protein content, but this was increased (p<0.05) when protease was added to the diets (0 to 10-d). Increasing the level of protease improved the pancreatic digestive enzymes, including trypsin (p<0.05), chymotrypsin (p<0.01), and general proteolytic enzymes (p<0.05).ConclusionThe commercial SBM could be replaced at up to 20% by RSBM for broilers. Although protease supplementation slightly improved the digestive enzymes, and the ileal digestibilities of CP and AA, the CP and AA were negatively affected by increasing RSBM.
Highlights
Soybean meal (SBM) is an excellent feed ingredient, it tends to fluctuate in supply and price in many parts of the world [1,2]
Pancreatic and jejunal tissue sampling On day 10th and 24th, the entire pancreas was collected from the sampled birds, snap-frozen in liquid nitrogen and stored in a freezer (–20°C) until analysis for tissue protein content and the activities of digestive enzymes
When increasing the replacement of commercial soybean meal (SBM) by raw soybean meals (RSBM) from 0 to 20%, the body weight (BW) was reduced by 6.0% (10 d) and reduced by 3.0% in both at d 24 and 35, whereas, the relative weight of pancreas was increased by 23.9%, 32.4%
Summary
Soybean meal (SBM) is an excellent feed ingredient, it tends to fluctuate in supply and price in many parts of the world [1,2]. Full-fat SBM can be prepared either after being treated by heat or from the raw soybean seed the latter contains anti-nutritional factors (ANF) [5,6]. Among the ANF, the most important one, in raw soybean meals (RSBM) is trypsin inhibitor (TI) [7,8]. The TIs in soybeans are a type of protein that can significantly reduce the digestibility and utilisation of proteins and amino acids (AAs) [9,10,11]. Protein digestibility could be reduced by a multifaceted action of ANF, such as by binding various nutrients and increasing the gut viscosity [12]. Due to the activity of TI, nitrogen retention can be negatively affected, and metabolic nitrogen excretion is increased [8,13]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
