Abstract
The antimicrobial activity of Lactobacilli has been widely exploited for prevention offood –borne pathogens e.g.: Escherichia coli being the major cause of diarrhea especially inchildren, because of bacteriocin activity and the importance of herbal drugs, hence this studywas designed to evaluate the synergistic effect of plant extract and bacteriocin produced byLactobacillus on the growth and pathogenesis of Enteropathogenic Ecoli.1. The Plantaricin production was induced by adding the mutagenic agent Mitomycin C.2. Purification of Plantaricin was made by heating crude plantaricin at 80ºC for 10min andthen purified by two steps method including extraction with n-butanol followed by gelfiltration chromatography on Sepharose 6B column. The results showed that the specificactivity was 1600 AU/mg protein with 8 purification folds and 12% recovery yield.3. The antibacterial activity of Quercus infectoria with concentration 300 mg/ml wasshowed highly antibacterial activity in vitro and in vivo.4. The result showed synergistic effect of Plantaricin with Quercus infectoria extract afterexperimental infection that induced by orally dosing with Escherichia coli in vivo. A result ofhistopathological study was recorded recovery of tissue.
Highlights
Bacteriocins are extracellularly released peptides or proteinaceous antimicrobial compounds, which exhibit a bactericidal effect against closely related bacteria
Purification of Plantaricin was made by heating crude plantaricin at 80oC for 10min and purified by two steps method including extraction with n-butanol followed by gel filtration chromatography on Sepharose 6B column
The results showed that the specific activity was 1600 AU/mg protein with 8 purification folds and 12% recovery yield
Summary
Bacteriocins are extracellularly released peptides or proteinaceous antimicrobial compounds, which exhibit a bactericidal effect against closely related bacteria. Bacteriocins of lactic acid bacteria are considered as safe natural preservatives or biopreservatives, as it is assumed that they are degraded by the proteases in gastro intestinal tract .Several types of bacteriocins from food-associated lactic acid bacteria have been identified and characterized, of which the important ones are Nisin, Bacteriocin, Diplococcin, Acidophilin, Bulgaricin, Helveticins, Lactacins and Plantaricins. The bactericidal activity of bacteriocins is attributable to destabilization of the functions of the cytoplasmic membrane of the target cells, altering the permeability properties of the membrane. Lactobacillus species are primarily used as probiotics, but can be used as starter cultures in various fermented foods. The galls have been reported to possess activities such as anti-diabetic, antibacterial, antiviral, antifungal, larvicidal, anti-inflammatory, anti-amoebic, and wound healing (3).Escherichia coli is one of the most common causes of morbidity and mortality in children with diarrhea all over the world in developing countries .(6)
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