Abstract

Biohydrogen production technology by fermentation uses biomass as raw materials to produce the renewable hydrogen energy. H2-producing bacteria(HPB) plays an important part in Biohydrogen production technology by fermentation。In this paper study on growth and Hydrogen production characteristics of HPB B49.Research has shown:⑴.Glucose concentration 10 g/L was the fittest substrate concentration of HPB B49 growth and hydrogen production;⑵Enzyme system growth of hydrogen-producing bacterium B49 at different growth phases influences hydrogen production;.⑶35 was the fittest temperature for cell growth and hydrogen production;⑷The fittest growth pH for hydrogen producing bacterium B49 was 4.5, while that for cell hydrogen production was 4.0;(5). This bacterium strain could be used as engineering bacterium in the different substrate hydrogen-producing experiments.

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