Growth and exuvial loss during larval and early juvenile development of the hermit crab Pagurus bernhardus reared in the laboratory

Abstract

Larvae of the hermit crab Pagurus bernhardus L. were obtained from origenerous females collected in 1986 near Helgoland (North Sea) and reared under constant conditions in the laboratory from hatching through the first juvenile instar. In regular intervals (every 1 or 2 d), changes in biomass during individual moult cycles were measured in dry weight (W), carbon (C), nitrogen (N) and hydrogen (H). Growth patterns in subsequent instars are described by regression equations. The megalopa does not eat and consequently looses biomass during its development. whereas all other instars gain biomass. Both instantaneous ineividual and instantaneous weight-specific growth rates decrease, in general, during the course of a moult cycle. As an exception, the megalopa was found to loose a constant fraction (ca 3 to 4% d-1) of its organic compounds. The C/N ratio indicates that this loss is primarily due to lipid catabolism. The term “secondary lecithotrophy” is proposed for non-feeding larval stages that develop with energy reserves accumulated by preceding feeding stages (in contrast to “primary lecithotrophy” of early stages that depend on yolk reserves from the egg). It is interpreted as an adaptation to an extremely specialized life style (here, life in a gastropod shell) requiring a particularly careful habitat selection before metamorphosis. As energetic costs of this adaptation, the megalopa loses ca one half of C and one third of N produced by the zocal instars combined. Relative elemental composition (C, N, H. as % of W) reveals a cyclic pattern with low postmoult and high premoult values during zocal development. The megalopa, in contrast, shows decreasing, and the juvenile rather constant, values. Regressions are given that describe average relations between W, C, N and H, so that conversions are possible between different measures of biomass. Exuvial losses (in μg W, C, N, H ind-1) increase in successive instars in an exponential manner. As a percentage of late premoult matter, or of the amounts produced during a given instar, exuvial losses of P. bernhardus zocae are very low as compared to other decapod larvae, but these losses increase significantly in later developmental stages. Elemental composition shows that the exuviae of successive instars contain increasing amounts of inorganic matter (ash), including inorganic C (carbonate).