Abstract
Abstract The growing interest of consumers to the new probiotic product has led the industry to focus on it. But viability of probiotic strain has a big challenge during shelf life of food. Therefore, to know the effect of food additives on survival of probiotic is important. The role of probiotics, garlic and yoghurt on the health of humans is widely known. In this study, we focus on finding answers to the question of whether selected beneficial bacteria will survive in the presence of ground garlic solution or fresh garlic juice and obtaining the growth parameters of that strains. Disk diffusion assays revealed that while Bifidobacterium longum BB536 is the most susceptible (49.37 ± 1.07 mm), Lactobacillus acidophilus 74-2 is the most resistant (no inhibition zone) bacteria to the fresh garlic juice, significantly. However, the strains were not adversely affected in possible usage rates of garlic in some Turkish appetizers. Enumeration assays also confirmed these unexpected findings. The addition of certain quantities of ground garlic or fresh garlic juice that did not show antimicrobial activity for selected probiotic strains are suitable for food matrix, especially yogurt based products.
Highlights
Probiotics are live microorganisms that confer a health benefit to the host when administered in adequate amounts (Food and Agriculture Organization, 2001)
Since there was no significant difference between triplicates for each bacteria strain, the growth curve data were obtained by taking mean values into account
We have demonstrated that, as expected, yoghurt and some probiotic strains have different growth parameters and abilities to decrease pH in same medium and temperature
Summary
Probiotics are live microorganisms that confer a health benefit to the host when administered in adequate amounts (Food and Agriculture Organization, 2001). The length of the lag phase can vary depending on the bacterial species, environmental conditions, and previous growth conditions. After this phase, cells divide exponentially, and this is known as the exponential phase. The growth rate slows down: most of the cells tend to divide, but death starts for others in the deceleration phase. As a result of the accumulation of metabolites and the lack of certain nutrition, cells die, a state called the death phase (Navarro Llorens et al, 2010)
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