Growth and development of hydatid cysts In immunised BALB/c mice with the lipopolysaccharide extracted from Klebsiella pneumoniae

Abstract

The study investigated the effect of the lipopolysaccharide (LPS) which was extracted from Klebsiella pneumoniae on immune response of BALB/c mice infected with secondary hydatid cyst of Echinococcus granulosus. The immunopathological changes accompanied with secondary hydatid disease in mice were followed in comparison with the positive control group (mice infected with hydatid disease but not activated with LPS) and negative control group (mice neither activated with LPS nor infected with hydatid disease) throughout (3-5) months period of the experiments. Criteria taken into consideration included the changes in numbers, weights and diameters of the hydatid cysts and the percentage of their reduction .In addition, changes in the weights of liver and spleen and their organ index. The results revealed an obvious decrease in numbers, weights and diameters of hydatid cysts grown in mice activated with LPS in comparison with the positive control group, in all experiments, supported by the reduction of the cyst number in activated mice. A decrease in weight and organic index of the liver and variation in the weight and organ index of spleen in activated mice. Therefore, it may be concluded that the lipopolysaccharide extracted from Klebsiella pneumoniae can be used as a non-toxic activator (stimulator) of the immune response against infection with secondary hydatid disease.