Abstract

Spores of Equisetum telmateia Ehrh. retained 74% viability after two years of storage under glycerine at minus 10 C. Stored spores germinated, grew, and formed mature gametangia on mineral nutrient media in petri plates and in micro-cultures where detailed cytological observations could be made. Growing gametophytes were favorable materials for studies of mitosis, chloroplast replication, gametogenesis, and senescence.

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