Growing Out of the Tropical Forests: Gene Flow of Native Mesoamerican Trees Among Forest and Mayan Homegardens

Abstract

This work aimed to evaluate domestication effects on the genetic structure of two dioecious speciesBrosimum alicastrumSw. (Moraceae) andSpondias purpureaL. (Anacardiaceae), and a heterostylous oneCordia dodecandraA. DC. (Cordiaceae), growing in remnant forests and homegardens within two climatic regions of the Peninsula of Yucatan. The trees ofB. alicastrumandC. dodecandraare propagated by seeds in both population types, while those ofS. purpureaare propagated asexually in the homegardens. ISSRs genetic markers were amplified from foliar tissue of 18 to 21 plants per population type/region combination for each species. Genetic diversity, genetic differentiation, and genetic structure estimators were obtained and compared among species at the regional and population level. We found higher polymorphism (37.5–41), but lower private alleles (4–4.4) and similar heterozygosity (0.1–0.12) in the species with sexual reproduction compared toS. purpurea(34, 8, and 0.11, respectively). Genetic diversity inB. alicastrumpopulations varied with the region; inC. dodecandra, to the population type; and inS. purpurea, to both the population type and the region. Unrestricted gene flow among regions was suggested by low ΦRTinC. dodecandraandS. purpurea(−0.006 and 0.002) but not forB. alicastrum(0.1). Gene flow between populations within the regions for the sexually reproducing species was suggested by lower θII(0.005–0.07 and 0.008–0.1) estimates than those ofS. purpurea(0.09 and 0.13). Even though the lowest paired FST(0.002–0.05) and ΦST(0.002–0.12) values were found between the northeastern forest and homegarden populations for the three species, the dendrogram, Bayesian assignment, and K-Means analyses suggest that the least differentiated populations are southwestern forest and homegarden populations ofB. alicastrumandS. purpurea, and the southwestern forest and northeastern homegarden ofC. dodecandra. The sexual reproduction, biotic interactions, and extensive management ofB. alicastrumandC. dodecandrain the agroforestry and the urban systems may contribute to connectivity between wild and domesticated populations, while inS. purpureathis connectivity is interrupted by the clonal propagation of the species in the homegardens.