Abstract
A new method of growing human embryonic neurons in glass capillary is proposed. Beside the simplicity of the method, the main advantages are represented by the possibility to study the interactions between a directional flow of human motile cells (leukocytes, red blood cells, sperm cells) and the neuronal monolayer and to evaluate the toxicity on neuronal growth of a copper wire or others filiform materials introduced in the glass capillary. The neurotoxicity of copper is verified by this device, because copper wire inserted into the capillary interferes with engraftment and growth of neuronal cells. No interference by neuronal monolayer was found with the flow of human sperms trough the capillary. Also the flow of leukocytes and erythrocytes occurred without agglutination or adhesion to cellular monolayer with serial observation at 30, 60, 120 minutes. A further advantage of this method is the possibility of withdrawing culture medium microquantities from capillary in order to study neuronal secretory activity.
Highlights
IntroductionThe neuronal monolayer culture can be performed in the traditional plastic flasks, alternatively other culture kits
The neuronal monolayer culture can be performed in the traditional plastic flasks, alternatively other culture kitsHow to cite this paper: Stabile, M. (2014) Growing Human Embryonic Neurons in Glass Capillary: A Simple Method to Study Interaction between Motile Cells and Neurons and to Explore the Neurotoxicity of Metals
The final leukocyte pellet was suspended in RPM1 and 0.5 cc of the final suspension was injected with insulin syringe in the glass capillary coated with neuronal monolayer
Summary
The neuronal monolayer culture can be performed in the traditional plastic flasks, alternatively other culture kits. How to cite this paper: Stabile, M. (2014) Growing Human Embryonic Neurons in Glass Capillary: A Simple Method to Study Interaction between Motile Cells and Neurons and to Explore the Neurotoxicity of Metals.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
