Group X secretory phospholipase A 2 induces potent productions of various lipid mediators in mouse peritoneal macrophages

Abstract

We have previously shown the expression of group X secretory phospholipase A 2 (sPLA 2-X) in mouse splenic macrophages and its powerful potency for releasing fatty acids from various intact cell membranes. Here, we examined the potency of sPLA 2-X in the production of lipid mediators in murine peritoneal macrophages. Mouse sPLA 2-X was found to induce a marked release of fatty acids including arachidonic acid and linoleic acid, which contrasted with little, if any, release by the action of group IB and IIA sPLA 2s. In resting macrophages, sPLA 2-X elicited a modest production of prostaglandin E 2 and thromboxane A 2. After the induction of cyclooxygenase-2 (COX-2) by pretreatment with lipopolysaccharide, a dramatic increase in the production of these eicosanoids was observed in sPLA 2-X-treated macrophages, which was completely blocked by the addition of either the specific sPLA 2 inhibitor indoxam or the COX inhibitor indomethacin. In accordance with its higher hydrolyzing activity toward phosphatidylcholine, mouse sPLA 2-X induced a potent production of lysophosphatidylcholine. These findings strongly suggest that sPLA 2-X plays a critical role in the production of various lipid mediators from macrophages. These events might be relevant to the progression of various pathological states, including chronic inflammation and atherosclerosis.