Griseococcin (1) from Bovistella radicata (Mont.) Pat and antifungal activity

Yong Ye,Qinghua Zeng,Qingmei Zeng

doi:10.1186/s12866-020-01961-x

Yong Ye, Qinghua Zeng + Show 1 more

Open Access

https://doi.org/10.1186/s12866-020-01961-x

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Journal: BMC microbiology	Publication Date: Sep 10, 2020
Citations: 2	License type: open-access

Affiliation: Hefei University of Technology

Abstract

BackgroundTo evaluate the antimicrobial and microbicidel activity of B. radicata fermentation broth, the broth was purified by DEAE-cellulose and sephadex LC-20 column. The compounds were submitted to spectral analyses (HPLC, FT-IR, 1D and 2D NMR etc.).ResultsThe purified compounds were identified as the Griseococcin(s) which were naphthoquinone derivatives, the Chemical formula and MW of Griseococcin (1) was determined as C37O10H43N and 661 Da. only Griseococcin (1) has good antimicrobial activity among the Griseococcin(s). The zone of inhibition (ZOI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC) of Griseococcin (1) were used to investigate the antimicrobial activity. Antifungal activity of Griseococcin (1) was significant, especially for main pathogenic fungus Trichophyton rubrum and Trichophyton mentagrophytes, MFC/MIC of Griseococcin (1) was 1, while MFC/MIC of postive control was greater than 4, the fungicidal effect of Griseococcin (1) was better than that of positive control.ConclusionsIn this paper, the secondary metabolite compound Griseococcin (1) from B. radicata was purified. The purified compound can restrain main pathogens (T. rubrum and T. mentagrophytes) leading to tinea pedis. The antifungal activity of Griseococcin (1) was similar to that of the positive control and the fungicidal effect of Griseococcin (1) was better than that of positive control, it might be suitable for pharmaceutical industries.

Highlights

To evaluate the antimicrobial and microbicidel activity of B. radicata fermentation broth, the broth was purified by DEAE-cellulose and sephadex LC-20 column
Petrović P, et al reported noticeable antimicrobial activity diversity for the methanol extracts obtained from Handkea utriformis (HU), H. excipuliformis (HE), and Vascellum pratense (VP) [21]
The aim of the present study was to evaluate the antimicrobial activity of Griseococcin (1) extracted from B. radicata fermentation broth

Summary

Introduction

To evaluate the antimicrobial and microbicidel activity of B. radicata fermentation broth, the broth was purified by DEAE-cellulose and sephadex LC-20 column. Patients that have tinea pedis may be affected by several pathogens, including filamentous fungi named Trichophyton rubrum and Trichophyton mentagrophytes [2], as well as a yeast named Candida albicans [3]. To treat tinea pedis, synthetic fungicides such as fluconazole, itraconazole, echinocandins [5], and miconazole nitrate, Puffballs are widely distributed in many provinces of China, and are various by more than 100 species [16]. Calvatia gigantean (Batsch ex Pers) Lloyd, Calvatia lilacina (Mont.et Berk.) Lloyd, Lasiosphaera fenzlii Reich, Lycoperdon pyriforme Schaeff.:pers, Bovistella radicata (Mont.) Pat, Handkea utriformis (HU), H. excipuliformis (HE), and Vascellum pratense (VP) are all common medicinal puffballs. Petrović P, et al reported noticeable antimicrobial activity diversity for the methanol extracts obtained from Handkea utriformis (HU), H. excipuliformis (HE), and Vascellum pratense (VP) [21].

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