Grip strength and metabolomic effects in mice fed a high‐nitrate beet diet (647.32)

Abstract

Beets (Beta vulgaris) are large edible tap roots vegetables belonging to the chenopod family. In addition to being a good source of essential micronutrients and minerals, beets also contain high levels of nitrates. Diets high in beet and nitrates have been demonstrated to improve exercise capacity, muscle contractility, and blood pressure. While these results have been replicated several times little is known about the mechanism of action. In this study we investigated grip strength and metabolomic changes in response to diets containing sodium nitrate, and beets providing nitrates at 5.5, 2.75, 1.37 mmol/g BW/day. Grip strength studies demonstrated a dose response improvement in the mice eating the beet diet, with a statistically significant peak grip strength improvement of 25%. These results are consistent with previous reports that beet juice improves muscle contractility. Untargeted metabolomic analysis in two separate studies indicated an upward trend (p=0.06) of plasma sphingosine 1‐phospate (S1P) concentrations in mice fed a beet diet (5.5mmol/gBW/day) compared with mice fed a control diet. Increased S1P has been previously correlated with exercise performance, muscle repair and activation of muscle stem cells. Overall these results suggest that high‐nitrate foods such as beets may improve muscle contractility through a mechanism of action which may include S1P and targets under its influence.