Grifola frondosa inhibits TNF‐α‐induced intestinal inflammation via suppression of MCP‐1 and IL‐8 expressions in HT‐29 human colon epithelial cells

Abstract

Grifola frondosa (GF), a king‐size edible mushroom, has been used as a health food for centuries in China and Japan. In the present study, we investigated an anti‐inflammatory action of GF in inflammatory bowel disease (IBD) by using HT‐29 human colon epithelial cells stimulated with TNF‐α to induce intestinal inflammatory condition in vitro. Treatment with GF significantly prevented the attachment of leukocytes to HT29 colon epithelial cells, one of the pathologic hallmarks in colon inflammation. The effect of GF was comparable to that of 5‐aminosalicylic acid, a commonly used drug in the treatment of IBD. In addition, pretreatment with GF inhibited TNF‐α‐induced MCP‐1 mRNA expression and protein secretion. Furthermore, GF reduced the TNF‐α‐induced interleukin‐8 mRNA expression. These results demonstrate that GF modulates intestinal inflammation via suppressing the expression of inflammatory cytokines, and suggest that GF may be a valuable medicinal food for prevention and/or attenuation of IBD.