Grey relational analysis on fingerprint of Vernonia anthelmintica extracts and its effect on proliferation of A375 human melanoma cells

Abstract

To establish the fingerprint of Vernonia anthelmintica extracts of different ethanol concentrations to study the effect of common peak components on the proliferation of A375 human melanoma cells and the correlation between fingerprint and pharmacodynamics of V. anthelmintica extracts, in order to provide both theoretical basis and data support for establishing a traditional Chinese medicine quality control mode with the combination of fingerprint and pharmacology. HPLC was applied to establish fingerprint of V. anthelmintica extracts of different ethanol concentrations and the similarity evaluation was made. MTT was used to study the proliferation of A375 human melanoma cells. Grey relation analysis was adopted to analyze the correlation between fingerprint and pharmacology of V. anthelmintica. The HPLC fingerprint of different V. anthelmintica ethanol extracts showed 13 common peaks with the proliferation effect on A375 human melanoma cells. The 4th peak had the largest contribution and highest correlation to cell proliferation. Different V. anthelmintica ethanol extracts have different fingerprints, which show a certain correlation between their common peaks and effect on cell proliferation.