Grewia asiatica Berry Juice Diminishes Anxiety, Depression, and Scopolamine-Induced Learning and Memory Impairment in Behavioral Experimental Animal Models.

Imran Imran,Hamid Saeed,Abdul Majeed,Sana Javaid,Faten Abdullah Alaqil,Noreen Samad,Muhammad Fawad Rasool,Faleh Alqahtani,Aroosa Waheed,Mohammed M Ahmed

doi:10.3389/fnut.2020.587367

Abstract

Grewia asiatica L. fruit natively called phalsa is a popular berry of Pakistan and widely consumed in the form of fresh juices and carbonated drinks in the summer season. The berry is enriched with antioxidants such as phenols, flavonoids, anthocyanins, and vitamin C. Scientifically, it is the least explored berry in terms of neuromodulatory activities, and therefore, in the designed study, chronically fed rats with the different dilutions (5%−30%) of fruit juice were subjected to behavioral assessment for anxiety, depression, and cognition (spatial memory) followed by biochemical analysis of isolated brains. Results revealed a prominent impact of 20 and 30% dilutions of fruit exudate as treated animals showed anxiolytic behavior to central zone (P < 0.05) of open field test (OFT) and open arms of elevated plus maze (EPM) (P < 0.05) in anxiety models. Overall, immobility of rats treated with a higher concentration of exudate in forced swim test (FST) was reduced (P < 0.05) presenting antidepressant-like activity. Moreover, in learning and memory experimental models, the treated animals reversed scopolamine-induced amnesic effects as evident from improved step-through latencies (P < 0.05 vs. scopolamine; passive avoidance test), spontaneous alternation behavior (P < 0.05 vs. scopolamine; Y-maze test), discrimination index (P < 0.05 vs. scopolamine; novel object recognition test), and escape latencies (P < 0.05 vs. scopolamine; Morris water maze). Biochemical studies of isolated brains from treated rats demonstrated significantly elevated levels of superoxide dismutase and glutathione peroxidase (P < 0.05), whereas levels of acetylcholinesterase and malondialdehyde level (P < 0.05) were reduced, indicating its potential to reduce oxidative damage in the brain and modulation with the cholinergic system. The outcomes of studies support the benefits of phytoconstituents possessed by G. asiatica fruit in the amelioration of neurological disorders that could be due to their antioxidative capacity or due to interaction with GABAergic, serotonergic, and cholinergic systems in the brain.

Highlights

It is well-known that healthy nutrition is an important key to healthy living
For studies affiliated with the learning and memory tasks, we introduced two new separate groups of rats such as group 6C and group 7
Total flavonoid contents calculated for all prepared extracts were ranging from 0.1 to 2.5 mg QE/g, and maximal content was discovered with 30% Gr.Ex, 2.3 ± 0.15 mg QE/g

Summary

Introduction

It is well-known that healthy nutrition is an important key to healthy living. The therapeutic importance of nutritional substances has been accepted for years. A recent survey reports that 33% of Americans tend to include those components in their daily diet that prove beneficial for their health as well [1]. Hippocrates’ philosophy claims that interventions made in a patient’s nutrition can help him recover from ailment [2]. Fruits include various nutrients that help promote health and protect against diseases [3]. When taken as a whole, they work best due to the synergistic effect of all of their unique constituents. The presence of several bioactive constituents in fruits tends to boost the overall health of consumers. That is why the fruit is designated as one of the most valuable nutritional sources rich in fiber, vitamins, carbohydrates, minerals, and antioxidants, which are crucial for a healthy lifestyle [4]

Methods

Results

Discussion

Conclusion