Abstract
Intestinal coccidiosis is a serious parasitic disease in poultry. Amprolium hydrochloride(AMP) is widely used for the treatment and prevention of coccidiosis. Three simple and green spectrophotometric methods have been developed for assay of AMP in bulk powder and in dosage form. The first method relies on measuring the first derivative (D1) spectrum at Δλ= 3 nm of AMP. This D1 spectrum possesses a characteristic peak at 234 nm. The second method relies on measuring the difference spectrophotometry (ΔA) and its first derivative (ΔD1) spectra of the drug solutions in 0.1 N HCl against the corresponding solutions in 0.1 N NaOH. The value of amplitudes of ΔA and ΔD1 (∆λ = 3 nm) were measured at 248 and 240 nm, respectively. The third method involves a colorimetric reaction with KMnO4 in alkaline medium and measuring the colored product at 610 nm. The developed methods were validated in accordance with ICH guidelines and evaluated for greenness using three greenness metrics; Analytical Eco-Scale (AES), Analytical GREEness calculator (AGREE), and Green Analytical Procedure Index (GAPI). All proposed methods showed excellent simplicity as they don't require sophisticated instrumentation or tedious analytical procedure steps.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
