Abstract
Two seedbox screening techniques were compared to determine levels of resistance of rice cultivars to three brown planthopper, Nilaparvata lugens (Stål), biotypes. The commonly used standard seedbox screening test (SSST) where eight second-instar nymphs were infested on 7-day-old seedlings was compared with a modification (MSST) where four second-instar nymphs were infested on each of 20-day-old plants. In the SSST, the initial infestation killed the susceptible cultivars whereas in the MSST the F 1 progeny killed the susceptible cultivars. The cultivars ASD 11, Wagwag, Utri Rajapan, IR46 and Kencana were susceptible in the SSST but moderately resistant or resistant in the MSST, indicating an increase in the level of resistance with plant age. Feeding rate and population growth of N. lugens were the factors involved in the resistance of 30-day-old plants. The MSST provides a rapid method for identifying field-resistant cultivars in the greenhouse and can be used to identify field-resistant lines in a rice-breeding programme for stable resistance to N. lugens.
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