Abstract

Previous work revealed significant variations in cannabinoid profiles ofCannabis sativa L. derived from a single seed source (P.1. 378939) and subjected to the same growth environment. Studies were conducted to evaluate the efficacy of propagation ofC. sativa by vegetative cuttings in order to increase uniformity of cannabinoid concentrations within a given plant population.C. sativa was successfully propagated by vegetative cuttings. However, there were both morphological and biochemical differences between seed-derived plants and their vegetative propagules. Delta-9-tetrahydrocannabinol concentrations were 4.1 times higher in vegetative propagules than in seed propagules. Vegetative cuttings also generally developed more profuse lateral branch growth; hence, foliage increased relative to their parent plants. Cannabinoid levels within the population of vegetative cuttings remained highly variable.

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