Abstract
In this study, novel approach for quantitative determination of eprosartan mesylate (EPR) and hydrochlorothiazide (HCTZ) by green UV absorbance ratio method. The choice of green solvents was based on GSK solvent selection guide, the green UV absorbance ratio method was used for quantitation of studied compounds in their pharmaceutical formulations. The aim of this work is to develop sensitive method to estimate HCTZ and EPR by using UV absorbance ratio method at 231 nm as isoabsorptive point (isosbestic point) and 271 nm as max wavelength of HCTZ, the method was validated according to ICH guidelines (Guideline, 2005).This method facilitates to any analyst in industrial field to quantify any formula containing EPR or HCTZ by precise, sensitive, reliable, accurate and fully validated method without use of hazardous toxic organic solvents. The method was simple, direct and no derivatization step was needed. The method was successfully applied to the analysis of studied drugs in their commercial pharmaceutical products.
Highlights
eprosartan mesylate (EPR) and HCTZ are important drugs used as antihypertensive medications
Determination of these APIs is of great importance in analytical field, many UV spectrophotometric methods are used for determination of EPR and HCTZ but rely on use of large amounts of toxic hazardous solvents and generate large amount of toxic hazardous waste products
The aim of this work is to develop sensitive method to estimate HCTZ and EPR by using UV absorbance ratio method at 231 nm as isoabsorptive point and 271 nm as max of HCTZ, the method was validated according to ICH guidelines
Summary
EPR and HCTZ are important drugs used as antihypertensive medications. Determination of these APIs is of great importance in analytical field, many UV spectrophotometric methods are used for determination of EPR and HCTZ but rely on use of large amounts of toxic hazardous solvents and generate large amount of toxic hazardous waste products. An absorbance ratio method have been developed for the estimation of EPR and HCTZ in pharmaceutical products (Anandakumar et al, 2011). Two new simple and selective assay methods have been presented for the analysis of EPR and HCTZ in pharmaceutical formulations. The first method is based on first-derivative ultraviolet spectrophotometry with zero-crossing measurements at 246 and 279 nm for EPR and HCT, respectively. The second method involved isocratic RP-HPLC (Hacioglu & Onal, 2012)
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